Differential Interference Contrast (DIC) microscopy is a technique that visualizes transparent samples by creating a three-dimensional image. It accomplishes this by splitting the light into two beams, which are then passed through the sample and recombined. The recombined light is then viewed through a polarizing filter, which makes the transparent structures appear with enhanced contrast.
DIC microscopy is often used to study cells and tissues, as it can provide detailed information about their structure and function. It is also used to study materials science, as it can reveal the presence of defects and other structural features.